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Federico Kalinec |
Research Overview
In the laboratory of Cell Structure and Function, we are exploring the function of the inner ear from two perspectives:
- The structure and function of the outer hair cells (OHC), which play a critical role in hearing as well as a protective role for inner hair cells.
- How to prevent life-saving drugs and noise from having a noxious effect on the inner ear and killing the outer hair cells.
The first of these objectives seeks a comprehensive understanding of the processes involved in the outer hair cell’s operation. Its intention is broad-based and will create a foundation for future pursuits in preventing damage, repairing and regenerating these structures. The second pursuit has a more immediate application: the development of intervention strategies to prevent damage to the outer hair cells.
Looking for achieving these goals, we concentrated our efforts in three major Projects: 1) Cytoskeletal Regulation of Outer Hair Cell Structure and Function, 2) Regulation of Inflammatory Responses in the Mammalian Cochlea, and 3) Drug- and Noise-induced Hearing Loss.
Selected Publications
Glucocorticoid-stimulated, transcription-independent release of annexin A1 by cochlear Hensen cells.
Kalinec F, Webster P, Maricle A, Guerrero D, Chakravarti DN, Chakravarti B, Gellibolian R, Kalinec G.
Br J Pharmacol. 2009 Dec;158(7):1820-34. Epub . PubMed PMID: 19912231; PubMed Central PMCID:
PMC2801223.
Pivotal role of Harakiri in the induction and prevention of gentamicin-induced hearing loss.
Kalinec GM, Fernandez-Zapico ME, Urrutia R, Esteban-Cruciani N, Chen S, Kalinec F.
Proc Natl Acad Sci U S A. 2005 Nov 1;102(44):16019-24. Epub 2005 Oct 20. PubMed PMID: 16239342; PubMed Central PMCID: PMC1276101.
Extraction of prestin-dependent and prestin-independent components from complex motile responses in guinea pig outer hair cells.
Matsumoto N, Kalinec F.
Biophys J. 2005 Dec;89(6):4343-51. Epub 2005 Sep 30. PubMed PMID: 16199492; PubMed Central PMCID: PMC1366998.
Structure of the cortical cytoskeleton inmammalian outer hair cells Holley MC, Kalinec F, Kachar B. J Cell Sci. 1992 Jul;102 ( Pt 3):569-80. PubMed PMID: 1506434.
A membrane-based force generation mechanism in auditory sensory cells. Kalinec F, Holley MC, Iwasa KH, Lim DJ, Kachar B. Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8671-5. PubMed PMID: 1528879; PubMed Central PMCID: PMC49982.
Cochlear outer hair cell bending in an external electric field. Frolenkov GI, Kalinec F, Tavartkiladze GA, Kachar B. Biophys J. 1997 Sep;73(3):1665-72. PubMed PMID: 9284333; PubMed Central PMCID: PMC1181065.
The membrane-based mechanism of cell motility in cochlear outer hair cells. Frolenkov GI, Atzori M, Kalinec F, Mammano F, Kachar B. Mol Biol Cell. 1998 Aug;9(8):1961-8. PubMed PMID: 9693359; PubMed Central PMCID: PMC25447.
Rho GTPases mediate the regulation of cochlear outer hair cell motility by acetylcholine. Kalinec F, Zhang M, Urrutia R, Kalinec G. J Biol Chem. 2000 Sep 8;275(36):28000-5. PubMed PMID: 10862776.
Structural microdomains in the lateral plasma membrane of cochlear outer hair cells. Zhang M, Kalinec F. J Assoc Res Otolaryngol. 2002 Sep;3(3):289-301. Epub 2002 Feb 27. PubMed PMID: 12382104.
Cisplatin-induced apoptosis in auditory cells: role of death receptor and mitochondrial pathways. Devarajan P, Savoca M, Castaneda MP, Park MS, Esteban-Cruciani N, Kalinec G, Kalinec F. Hear Res. 2002 Dec;174(1-2):45-54. PubMed PMID: 12433395.
A cochlear cell line as an in vitro system for drug ototoxicity screening. Kalinec GM, Webster P, Lim DJ, Kalinec F. Audiol Neurootol. 2003 Jul-Aug;8(4):177-89. PubMed PMID: 12811000.
ROCK-dependent and ROCK-independent control of cochlear outer hair cell electromotility. Zhang M, Kalinec GM, Urrutia R, Billadeau DD, Kalinec F. J Biol Chem. 2003 Sep 12;278(37):35644-50. Epub 2003 Jul 1. PubMed PMID: 12837763.
Prestin-dependent and prestin-independent motility of guinea pig outer hair cells. Matsumoto N, Kalinec F. Hear Res. 2005 Oct;208(1-2):1-13. Epub 2005 Jul 5. PubMed PMID: 16000248.
Acetaminophen ototoxicity after acetaminophen/hydrocodone abuse: evidence from two parallel in vitro mouse models. Yorgason JG, Kalinec GM, Luxford WM, Warren FM, Kalinec F. Otolaryngol Head Neck Surg. 2010 Jun;142(6):814-9, 819.e1-2. Epub 2010 Apr 9. PubMed PMID: 20493351.