Cryosection through a Listeria monocytogenes infected cell

Listeria monocytogenes infects cells through the phagocytic pathway. This micrograph shows a cryo-section through a listeria-containing phagosome. The bacterium is shown in green. The section has been labeled with antibodies that bind to a compound that accumulates in the lumen of subcellular organelles with acidic contents. The antibodies have been visualized with colloidal gold particles, shown in red. Clearly, during the early stages of infection, organelles with acidic contents fuse with the phagosome. Colorized with Adobe Photoshop.
Ahmanson Center for
Advanced Electron Microscopy and Imaging